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insert er-1 earphones  (Etymotic Research Inc)
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Etymotic Research Inc insert er-1 earphones
Insert Er 1 Earphones, supplied by Etymotic Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transwell inserts  (Servicebio Inc)
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Servicebio Inc transwell inserts
Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) <t>Transwell</t> assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).
Transwell Inserts, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transwell inserts/product/Servicebio Inc
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cvc insertions  (Kuang Lung Shing)
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Kuang Lung Shing cvc insertions
Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) <t>Transwell</t> assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).
Cvc Insertions, supplied by Kuang Lung Shing, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inserts  (Fisher Scientific)
86
Fisher Scientific inserts
Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) <t>Transwell</t> assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).
Inserts, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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temperature responsive cell culture inserts  (Cellseed Inc)
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Cellseed Inc temperature responsive cell culture inserts
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Temperature Responsive Cell Culture Inserts, supplied by Cellseed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mc polyethylene insert  (Zimmer Biomet)
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Zimmer Biomet mc polyethylene insert
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Mc Polyethylene Insert, supplied by Zimmer Biomet, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transwell tc inserts  (Sarstedt)
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Sarstedt transwell tc inserts
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Transwell Tc Inserts, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mucilairtm transwell inserts  (Epithelix)
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Epithelix mucilairtm transwell inserts
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Mucilairtm Transwell Inserts, supplied by Epithelix, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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specialized er1 insert earphones  (Etymotic Research Inc)
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Etymotic Research Inc specialized er1 insert earphones
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Specialized Er1 Insert Earphones, supplied by Etymotic Research Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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103 cells per insert  (Sarstedt)
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Sarstedt 103 cells per insert
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
103 Cells Per Insert, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

Journal: Bioactive Materials

Article Title: Reconstructing the ischemic osteogenic microenvironment through hierarchical scaffolds orchestrating Mg 2+ signaling and neuropilin-1–mediated angiogenesis

doi: 10.1016/j.bioactmat.2026.02.031

Figure Lengend Snippet: Evaluation of angiogenic potential induced by Mg 2+ and NRP-1. ( A ) Scratch assay in three different microenvironments. ( B ) Transwell assay in three different microenvironments. ( C ) Quantitative analysis of wound healing area. ( D ) Quantitative analysis of number of migration cells. ( E ) Immunofluorescence staining of VEGFA and FGF2, with DAPI for nuclear staining and F-actin for cytoskeleton labeling. ( F ) Tube formation evaluation in four different microenvironments. ( G ) Quantitative analysis of number of junction. ( H ) Quantitative analysis of Flu intensity. ( I ) Western-blot analysis of VEGFA, FGF2, and Nr4a1. ( J-L ) Quantitative analysis of relative protein expression of VEGFA, FGF2, and Nr4a1. Data are presented as mean values ± s.d. (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA).

Article Snippet: For the Transwell assay, BMSCs were seeded in the upper chambers of Transwell inserts (8.0 μm pore size; Servicebio, China), while different culture conditions were applied in the lower chambers according to the experimental groups.

Techniques: Wound Healing Assay, Transwell Assay, Migration, Immunofluorescence, Staining, Labeling, Western Blot, Expressing

Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

Journal: Regenerative Therapy

Article Title: Characterization of the role and fate of nasal mucosal epithelial cell sheets in middle ear regeneration

doi: 10.1016/j.reth.2026.101130

Figure Lengend Snippet: Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

Article Snippet: The cells were then seeded onto temperature-responsive cell culture inserts (CellSeed) at a density of 4 × 10 4 cells/cm 2 and cultured in KCM for 7 days.

Techniques: Derivative Assay, Incubation, Fluorescence, Microscopy, Immunohistochemical staining, Staining